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Reference Strain Atcc 27853 Rnd Efflux Pump Gene δct Value δδct Value Interpretation Atcc27853a P113 P118 T117 Xm8 P113 P118 T117 Xm8 Mexab Oprm, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rabbit anti p113 ampkα
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Demegen Inc histatin-5 amidated fragment p113
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ATCC p113 gene
SDS‒PAGE detection of recombinant Hsp70‒P113 protein expression and purification. M: protein marker; 1: uninduced pET-30a/BL21(DE3); 2: uninduced <t>pET-30a-rHsp70-P113/BL21(DE3);</t> 3: induced pET-30a-rHsp70-P113/BL21(DE3); 4: supernatant of the induced pET-30a-rHsp70-P113/BL21(DE3); 5: precipitate of induced pET-30a-rHsp70-P113/BL21(DE3); 6: purified recombinant protein Hsp70-P113
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SDS‒PAGE detection of recombinant Hsp70‒P113 protein expression and purification. M: protein marker; 1: uninduced pET-30a/BL21(DE3); 2: uninduced <t>pET-30a-rHsp70-P113/BL21(DE3);</t> 3: induced pET-30a-rHsp70-P113/BL21(DE3); 4: supernatant of the induced pET-30a-rHsp70-P113/BL21(DE3); 5: precipitate of induced pET-30a-rHsp70-P113/BL21(DE3); 6: purified recombinant protein Hsp70-P113
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Pacgen Life afp peptide p113
SDS‒PAGE detection of recombinant Hsp70‒P113 protein expression and purification. M: protein marker; 1: uninduced pET-30a/BL21(DE3); 2: uninduced <t>pET-30a-rHsp70-P113/BL21(DE3);</t> 3: induced pET-30a-rHsp70-P113/BL21(DE3); 4: supernatant of the induced pET-30a-rHsp70-P113/BL21(DE3); 5: precipitate of induced pET-30a-rHsp70-P113/BL21(DE3); 6: purified recombinant protein Hsp70-P113
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Greiner Bio 96-well u-bottom sterile plate greiner bio-one #6018-p113
SDS‒PAGE detection of recombinant Hsp70‒P113 protein expression and purification. M: protein marker; 1: uninduced pET-30a/BL21(DE3); 2: uninduced <t>pET-30a-rHsp70-P113/BL21(DE3);</t> 3: induced pET-30a-rHsp70-P113/BL21(DE3); 4: supernatant of the induced pET-30a-rHsp70-P113/BL21(DE3); 5: precipitate of induced pET-30a-rHsp70-P113/BL21(DE3); 6: purified recombinant protein Hsp70-P113
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SDS‒PAGE detection of recombinant Hsp70‒P113 protein expression and purification. M: protein marker; 1: uninduced pET-30a/BL21(DE3); 2: uninduced pET-30a-rHsp70-P113/BL21(DE3); 3: induced pET-30a-rHsp70-P113/BL21(DE3); 4: supernatant of the induced pET-30a-rHsp70-P113/BL21(DE3); 5: precipitate of induced pET-30a-rHsp70-P113/BL21(DE3); 6: purified recombinant protein Hsp70-P113

Journal: BMC Veterinary Research

Article Title: Investigating the immunological activity of the Hsp70-P113 fusion protein for Mycoplasma ovipneumoniae detection: a groundbreaking study

doi: 10.1186/s12917-024-04274-7

Figure Lengend Snippet: SDS‒PAGE detection of recombinant Hsp70‒P113 protein expression and purification. M: protein marker; 1: uninduced pET-30a/BL21(DE3); 2: uninduced pET-30a-rHsp70-P113/BL21(DE3); 3: induced pET-30a-rHsp70-P113/BL21(DE3); 4: supernatant of the induced pET-30a-rHsp70-P113/BL21(DE3); 5: precipitate of induced pET-30a-rHsp70-P113/BL21(DE3); 6: purified recombinant protein Hsp70-P113

Article Snippet: The Protean program in DNAStar 7.0 software was utilized to analyse the secondary structures of the Hsp70 gene (FJ-29 strain, GenBank accession number: OP121620) and the P113 gene (ATCC 29419 strain, GenBank accession number: KR021380) of Movi, with the aim of identifying prominent antigenic regions.

Techniques: Recombinant, Expressing, Purification, Marker

Western blot analysis of the reactogenicity of the recombinant protein Hsp70-P113. 1: Anti-His tag antibody as the primary antibody; 2: recombinant protein hyperimmune serum as the primary antibody (hyperimmune serum prepared by immunizing BALB/c mice with purified Hsp70-P113 recombinant protein); 3: Movi hyperimmune serum as the primary antibody (hyperimmune serum prepared by immunizing BALB/c mice with Movi whole bacterium antigen); 4. The serum of nonimmunized mice (blank control) was used as the primary antibody. M: protein marker. 1–4:HRP-labelled goat anti-mouse IgG was used as the secondary antibody

Journal: BMC Veterinary Research

Article Title: Investigating the immunological activity of the Hsp70-P113 fusion protein for Mycoplasma ovipneumoniae detection: a groundbreaking study

doi: 10.1186/s12917-024-04274-7

Figure Lengend Snippet: Western blot analysis of the reactogenicity of the recombinant protein Hsp70-P113. 1: Anti-His tag antibody as the primary antibody; 2: recombinant protein hyperimmune serum as the primary antibody (hyperimmune serum prepared by immunizing BALB/c mice with purified Hsp70-P113 recombinant protein); 3: Movi hyperimmune serum as the primary antibody (hyperimmune serum prepared by immunizing BALB/c mice with Movi whole bacterium antigen); 4. The serum of nonimmunized mice (blank control) was used as the primary antibody. M: protein marker. 1–4:HRP-labelled goat anti-mouse IgG was used as the secondary antibody

Article Snippet: The Protean program in DNAStar 7.0 software was utilized to analyse the secondary structures of the Hsp70 gene (FJ-29 strain, GenBank accession number: OP121620) and the P113 gene (ATCC 29419 strain, GenBank accession number: KR021380) of Movi, with the aim of identifying prominent antigenic regions.

Techniques: Western Blot, Recombinant, Purification, Control, Marker